Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Expression of IL-4/IL-13 receptors in differentiating human airway epithelial cells

doi: 10.1152/ajplung.00422.2009

Figure Lengend Snippet: A: localization of IL-13Rα1 in primary AEC grown in submersion culture or ALI culture for either 1 or 3 wk, as demonstrated by confocal microscopy. In each series, cells were labeled for the cell subtype marker as noted (green color) and for the receptor subunit as noted (red color). Yellow represents overlap (colocalization). Background was calculated from cells in which labeling omitted the primary antibody (negative controls) and then was subtracted from the display range for all images shown. Bar, 20 μm for all images. B: representative Western blot and densitometry of Western blots from protein lysates collected from AEC grown in submersion culture and in ALI culture for 1 or 3 wk. Densitometry was normalized to GAPDH abundance assessed in the same blots. n = 3 Experiments.

Article Snippet: For antibodies in Western blots, a rabbit polyclonal anti-IL-4Rα was obtained from Strategic Diagnostics (Newark, DE), a goat monoclonal anti-IL-13Rα2 was obtained from R&D Systems (Minneapolis, MN), and mouse monoclonal anti-IL-13Rα1, anti-IL-2Rγc, and anti-GAPDH were obtained from Abcam.

Techniques: Confocal Microscopy, Labeling, Marker, Western Blot

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Expression of IL-4/IL-13 receptors in differentiating human airway epithelial cells

doi: 10.1152/ajplung.00422.2009

Figure Lengend Snippet: A: localization of IL-2Rγc in primary AEC grown in submersion culture or ALI culture for either 1 or 3 wk, as demonstrated by confocal microscopy. In each series, cells were labeled for the cell subtype marker as noted (green color) and for the receptor subunit as noted (red color). Yellow represents overlap (colocalization). Background was calculated from cells in which labeling omitted the primary antibody (negative controls) and then was subtracted from the display range for all images shown. Bar, 20 μm for all images. B: representative Western blot and densitometry of Western blots from protein lysates collected from AEC grown in submersion culture and in ALI culture for 1 or 3 wk. Densitometry was normalized to GAPDH abundance assessed in the same blots. n = 3 Experiments.

Article Snippet: For antibodies in Western blots, a rabbit polyclonal anti-IL-4Rα was obtained from Strategic Diagnostics (Newark, DE), a goat monoclonal anti-IL-13Rα2 was obtained from R&D Systems (Minneapolis, MN), and mouse monoclonal anti-IL-13Rα1, anti-IL-2Rγc, and anti-GAPDH were obtained from Abcam.

Techniques: Confocal Microscopy, Labeling, Marker, Western Blot